FLOW-INJECTION ANALYSIS WITH FOURIER-TRANSFORM INFRARED DETECTION FORCLINICAL AND PROCESS ANALYSIS

As the analysis of glucose and urea is of special interest in the fiel d of clinical and process analysis, an enzymatic FIA-FTIR system is pr esented, based on the reactions of glucose to gluconic acid using gluc ose oxidase as well as of urea to ammonium carbonate catalyzed by urea se. The method is tested with standard solutions, with samples simulat ing the blood composition and with a variety of fruit juices and soft drinks. Whereas the systematic error is within few percent, as conclud ed from the slope of the calibration graph, the reproducibility of the method is up to now not satisfactory for routine applications in proc ess analysis and has to be improved.