H. Mascher et M. Wasilewski, SIMPLE AND FAST HPLC METHOD FOR THE DETERMINATION OF TRIAMTERENE AND HYDROXYTRIAMTERENESULPHATE IN PLASMA AND URINE, Journal of liquid chromatography, 17(7), 1994, pp. 1577-1585
Determination of triamterene (T) and hydroxytriamterenesuIphate (HTS)
in plasma and urine requires a very sensitive and selective method. As
both of the substances are highly hydrophilic, it is hardly feasible
to carry out an extraction. The method described obviates the need for
pre-cleansing of samples. Plasma and urine samples, diluted with wate
r, are directly injected into the HPLC-column and analysed. For the de
tection of both of the substances fluorescence was used. The use of a
Spherisorb-NH2-column with a mobile phase, consisting only of a buffer
solution in water, makes it possible to dispense with protein precipi
tation of plasma. Both substances were analysed within 2 minutes in a
single run. Detection limits of 1 ng T and 20 ng HTS/ml plasma as requ
ired in practice, were obtained without any difficulties. Referring to
the precision of this method with plasma samples, the variation coeff
icient was below 3 % with HTS in the range of 20 to 1100 ng HTS/ml. Da
y to day variation showed with T in plasma values of smaller than 7 %
in the range of 1 to 100 ng/ml.