DETERMINATION OF BENZIODARONE IN HUMAN PLASMA AND TABLETS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A rapid HPLC method is described for benziodarone assay in human plasm a using a LiChrosorb RP-18 column and UV detection at 240 nm. Protein precipitation is followed by extraction of benziodarone and khellin (i nternal standard). Extraction of the active substances with diethyl et her at pH ca. 4.8 ensures fairly good recovery (91.04%, mean). Detecti on limit for this method of determination is 20 ng using a 1 ml sample . The method id specific and can also be used for the determination of benziodarone in pharmaceuticals (tablets).