SELECTIVE ISOLATION OF POLIOVIRUS IN RECOMBINANT MURINE CELL-LINE EXPRESSING THE HUMAN POLIOVIRUS RECEPTOR GENE

Sixty-eight laboratory strains representing 49 enterovirus, 10 adenovi rus, and 3 reovirus serotypes were inoculated in a recombinant murine cell line expressing the human poliovirus receptor gene (L alpha cells ). Only polioviruses caused cytopathic effect over a 10-day period. Li kewise. only polioviruses were isolated, by use of Lot cells, from 168 fecal specimens from children from developing countries. These result s suggest that the recombinant L alpha cells can be used for selective isolation of poliovirus from clinical specimens.