EFFECTIVE USE OF FROZEN DONOR PERIPHERAL-BLOOD MONONUCLEAR-CELLS FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATION FROM VERTICALLY INFECTEDPEDIATRIC-PATIENTS
Mo. Paul et al., EFFECTIVE USE OF FROZEN DONOR PERIPHERAL-BLOOD MONONUCLEAR-CELLS FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATION FROM VERTICALLY INFECTEDPEDIATRIC-PATIENTS, Journal of clinical microbiology, 32(5), 1994, pp. 1379-1382
In this study, we examined variables related to human immunodeficiency
virus (HIV) isolation utilizing samples from 51 HIV-infected (153 pla
sma and 122 peripheral blood mononuclear cell PBMC samples) and 57 u
ninfected (182 plasma and 163 PBMC samples) infants. Our chief observa
tion was that cryopreservation of donor PBMCs does not significantly a
lter their sensitivity or specificity for isolation of HIV from patien
t PBMCs or plasma.