EFFECTIVE USE OF FROZEN DONOR PERIPHERAL-BLOOD MONONUCLEAR-CELLS FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATION FROM VERTICALLY INFECTEDPEDIATRIC-PATIENTS

Citation
Mo. Paul et al., EFFECTIVE USE OF FROZEN DONOR PERIPHERAL-BLOOD MONONUCLEAR-CELLS FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATION FROM VERTICALLY INFECTEDPEDIATRIC-PATIENTS, Journal of clinical microbiology, 32(5), 1994, pp. 1379-1382
Citations number
27
Categorie Soggetti
Microbiology
ISSN journal
00951137
Volume
32
Issue
5
Year of publication
1994
Pages
1379 - 1382
Database
ISI
SICI code
0095-1137(1994)32:5<1379:EUOFDP>2.0.ZU;2-9
Abstract
In this study, we examined variables related to human immunodeficiency virus (HIV) isolation utilizing samples from 51 HIV-infected (153 pla sma and 122 peripheral blood mononuclear cell PBMC samples) and 57 u ninfected (182 plasma and 163 PBMC samples) infants. Our chief observa tion was that cryopreservation of donor PBMCs does not significantly a lter their sensitivity or specificity for isolation of HIV from patien t PBMCs or plasma.