In vivo canine experiments have demonstrated that vocal fold stiffness
varies proportionately with changing levels of recurrent laryngeal ne
rve (RLN) and superior laryngeal nerve (SLN) stimulation. This study e
valuated the morphologic changes in the glottis at varying levels of n
erve stimulation and the presumed effects on laryngeal air particle ve
locity. Stroboscopic data from the in vivo canine model of phonation w
ere examined under varying conditions of RLN and SLN stimulation. Comp
uterized analysis of stroboscopic images was used to reconstruct the g
lottal area vs. time waveforms. As RLN stimulation increased, glottal
area per cycle decreased (p < 0.05). However, as SLN stimulation incre
ased, glottal area per cycle increased (p < 0.05). These results suppo
rt the hypothesis that increasing RLN stimulation at similar levels of
SLN stimulation produces an increase in air particle velocity, wherea
s an increase in SLN stimulation causes a decrease in air particle vel
ocity.