We determined the antibody prevalence to Ehrlichia spp., in white-tail
ed deer (Odocoileus virginianus) and the geographic distribution of se
ropositive animals in 84 counties in Alabama, Arkansas, Florida, Georg
ia, Illinois, Kentucky, Louisiana, Maryland, Massachusetts, Mississipp
i, Missouri, North Carolina, South Carolina, Tennessee, Texas, Virgini
a, and West Virginia (USA). Using an indirect fluorescent antibody tes
t we detected antibodies (greater than or equal to 1:128) to this bact
erium in 544 (43%) of 1269 deer. Presence of antibodies to Ehrlichia s
pp, was related to a southerly latitude, low elevation, and resulting
milder climatic conditions. It appears that white-tailed deer were nat
urally infected with Ehrlichia spp.; the infection was widely distribu
ted throughout the southeastern United States. Based on these data, we
propose that white-tailed deer play a role in the natural history of
Ehrlichia spp. infection in the United States.