LIP-MODEL-BASED 3-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF HIV-INFECTED ENTIRE CELLS

Citation
P. Gremillet et al., LIP-MODEL-BASED 3-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF HIV-INFECTED ENTIRE CELLS, Journal of Microscopy, 174, 1994, pp. 31-38
Citations number
16
Categorie Soggetti
Microscopy
Journal title
ISSN journal
00222720
Volume
174
Year of publication
1994
Part
1
Pages
31 - 38
Database
ISI
SICI code
0022-2720(1994)174:<31:L3RAVO>2.0.ZU;2-M
Abstract
This paper presents a global solution from acquisition to visualizatio n for the three-dimensional reconstruction of cell sections. Original techniques are proposed for the correct handling of the geometrical se ction distortions, and a new interpretation based on the logarithmic i mage processing (LIP) model is applied in order to create normalized g rey-level sections where these are missing. Finally, a new method for generating a mesh of triangles to describe the envelope of the reconst ructed cell is proposed, as well as a visualization mixing image synth esis and grey-level information. The product allows the user to explor e the reconstructed cellular block in any desired direction, by showin g user-defined grey-level sections inside the block mixed to a synthet ic view of the cell envelope.