N. Cochet et al., ICE NUCLEATION BY BACTERIA - PRODUCTION AND ACTIVITY, Colloids and surfaces. A, Physicochemical and engineering aspects, 83(3), 1994, pp. 187-191
The ability of Pseudomonas syringae to catalyse ice formation at relat
ively high temperatures from - 2 to - 5-degrees-C has received conside
rable attention.A fermentation method was used in order to obtain a hi
gh nucleation activity expressed in ice nuclei per cell. This was base
d on the optimization of the medium composition and control of the fer
mentation parameters. Meat peptone medium appeared to provide the best
conditions for biomass production and ice nucleation activity. Moreov
er, we observed a positive influence on the nucleation activity due to
Mn2+ ion addition. The pH control of the culture seemed to influence
negatively the bacterial nucleation activity.