COMBINATION OF COBALT LABELING WITH IMMUNOCYTOCHEMICAL REACTIONS FOR ELECTRON-MICROSCOPIC INVESTIGATIONS ON FROG SPINAL-CORD

Cobalt staining of primary afferents in frog spinal cord was combined with peroxidase-antiperoxidase pre-embedding or immunogold post-embedd ing immunocytochemical labelling. Our results have shown that cobalt l abelling can easily be distinguished from both of the immunoreaction e nd products. The protocol of cobalt labelling did not affect the immun oreactivity of structures. The morphology and synaptology of cobalt la belled and immunostained profiles in our sections were very similar to those reported in previous studies using different double labelling t echniques. These results indicate that this new combined method could be used as an alternative double labelling technique in electron micro scopic studies on nervous tissues. (C) 1994 Wiley-Liss, Inc.