ISOLATION OF SITE-SPECIFIC ENDONUCLEASE AND METHYLASE FROM BACILLUS-CEREUS-83

Authors
MATVIENKO NN KRAMAROV VM ZHELEZNAYA LA MATVIENKO NI
Citation
Nn. Matvienko et al., ISOLATION OF SITE-SPECIFIC ENDONUCLEASE AND METHYLASE FROM BACILLUS-CEREUS-83, Biochemistry, 58(12), 1993, pp. 1357-1369
Citations number
15
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
58
Issue
12
Year of publication
1993
Pages
1357 - 1369
Database
ISI
SICI code
0006-2979(1993)58:12<1357:IOSEAM>2.0.ZU;2-U
Abstract
Site-specific endonuclease R Bce83I and methylase M Bce83I were isolat ed from Bacillus cereus 83 by three consecutive chromatographies on bl ue agarose, hydroxyapatite, and heparin-Sepharose. R Bce83I recognizes the (5'-CTTGAG16N-3'down arrow)(3'-GAACTC14N-5'up arrow) Sequence on the DNA and cleaves the DNA as indicated by the arrows. The endonuclea se is stimulated by S-adenosyl-L-methionine. Therefore, it is a type I V restriction enzyme.