A. Hirata et al., MONOCLONAL-ANTIBODY TO PROTEOGLYCAN DERIVED FROM GRIFOLA-FRONDOSA (MAITAKE), Biological & pharmaceutical bulletin, 17(4), 1994, pp. 539-542
A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c m
ice with a proteoglycan fraction derived from Grifola frondosa (Maitak
e mushroom), followed by the hybridization of spleen cells with mouse
myeloma cells. The MAb (subclass; Ig G(2b)), designated MPG2, reacted
with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentina
n, but not with dextran, pullulan, mannan and xylan. Immunohistochemis
try (ABC-GO method) shelved that MAb MPG2 reacted with lysosomal prote
oglycan and (1->6)-beta-branched laminaritriose taken up by rabbit per
itoneal macrophages. These results suggest that this MAb may recognize
mainly (1->3)-beta-D-glucan, and may be useful for determining the im
munological properties of Grifola frondosa-derived proteoglycan.