MONOCLONAL-ANTIBODY TO PROTEOGLYCAN DERIVED FROM GRIFOLA-FRONDOSA (MAITAKE)

A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c m ice with a proteoglycan fraction derived from Grifola frondosa (Maitak e mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G(2b)), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentina n, but not with dextran, pullulan, mannan and xylan. Immunohistochemis try (ABC-GO method) shelved that MAb MPG2 reacted with lysosomal prote oglycan and (1->6)-beta-branched laminaritriose taken up by rabbit per itoneal macrophages. These results suggest that this MAb may recognize mainly (1->3)-beta-D-glucan, and may be useful for determining the im munological properties of Grifola frondosa-derived proteoglycan.