IDENTIFICATION OF THE MALTOSE TRANSPORT PROTEIN OF SACCHAROMYCES-CEREVISIAE

Identification of the maltose transport protein of Saccharomyces cerev isiae was attempted by searching for maltose-inducible proteins in iso lated plasma membranes. Membranes from maltose-grown cells contained t wo proteins that were absent in glucose-grown cells. The proteins diff ered in size, but peptide sequence analysis indicated a high degree of homology. The amino-terminal and internal sequences of the largest pr otein, with an apparent molecular mass of 64 kDa, were determined. The se sequences were identical to predicted amino acid sequences in the M AL61 gene product. It is concluded that this protein is the inducible maltose permease of Saccharomyces cerevisiae. (C) 1994 Academic Press, Inc.