A CHICKEN TRANSFERRIN-BINDING PROTEIN IS HEAT-SHOCK PROTEIN-108

A previously described transferrin binding protein from chicken oviduc t has been identified as a heat shock protein, HSP108, by microsequenc ing of RP-HPLC purified tryptic peptides. The protein purified from ov iduct by SDS-PAGE or from liver by ovotransferrin-affinity chromatogra phy reacts with a monoclonal antibody raised against HSP108. The cDNA sequence predicts a KDEL peptide at the carboxyl terminus, but the pro tein does not react with anti-KDEL monoclonal antibodies, suggesting t hat HSP108 is processed in a way that eliminates the KDEL epitope. (C) 1994 Academic Press, Inc.