TRANSCRIPTION OF LIGNINASE H8 BY PHANEROCHAETE-CHRYSOSPORIUM UNDER NUTRIENT NITROGEN SUFFICIENT CONDITIONS

Dot blot analyses showed that more ligninase H8 mRNA was present in am monium sufficient cultures of Phanerochaete chrysosporium than in ammo nium limited cultures. Reverse transcription followed by polymerase ch ain reaction and DNA sequencing verified that H8 mRNA was present unde r both conditions. Fast protein liquid chromatography profiles indicat ed that H8 was present but lacked heme in ammonium sufficient cultures . These data indicated that H8 was transcribed and translated by day 5 under ammonium sufficient conditions, but was inactive due to lack of heme. (C) 1994 Academic Press, Inc.