CHARACTERIZATION AND SEQUENCING OF AN UNCOUPLED LACTOSE CARRIER MUTANT OF ESCHERICHIA-COLI

A lactose carrier mutant of Escherichia coli (ML308-22) showed a sever e defect in thiomethylgalactoside accumulation but a faster than norma l entry of o-nitrophenyl-galactoside. Sequencing of the mutant lacY ge ne revealed a point mutation resulting in the substitution of glycine- 159 by a cysteine residue. The mutant showed an increased sensitivity to sulfhydryl reagents, a property that is consistent with the view th at the Cysteine-159 is in or near the sugar recognition site and the e nergy coupling region of the carrier. (C) 1994 Academic Press, Inc.