Dw. Morel, REDUCED CHOLESTEROL EFFLUX TO MILDLY OXIDIZED HIGH-DENSITY-LIPOPROTEIN, Biochemical and biophysical research communications, 200(1), 1994, pp. 408-416
Mild oxidation (<6h) of human high density lipoprotein (HDL) induced b
y incubation with copper ions increased its thiobarbituric acid reacti
vity, decreased its phospholipid content, increased its free cholester
ol to phospholipid ratio and reduced its ability to mediate cholestero
l efflux from cells. While protein modification did occur with further
oxidation (>12h), diminished efflux of cell cholesterol was apparant
for HDL samples without increased electrophoretic mobility or altered
apolipoproteins. Thus, reduced cholesterol efflux to mildly oxidized H
DL may reflect changes in its lipids rather than its apoproteins. By l
imiting cholesterol removal from cells, any mild oxidation of HDL whic
h might occur in vivo could contribute to cellular cholesterol accumul
ation. (C) 1994 Academic Press, Inc.