DIFFERENTIAL EXPRESSION OF VIRAL AND HUMAN INTERLEUKIN-10 (IL-10) BY PRIMARY B-CELL TUMORS AND B-CELL LINES

Human and viral interleukin-10 (IL-10) possess growth factor activity for human B cells and may act as autocrine growth factors in B cell ma lignancies. To study this possibility we have measured viral (v) and h uman (h) IL-10 expression in EBV-positive and negative B lineage tumor s and tumor cell lines. Previous studies demonstrated IL-10 expression in cell lines and now we describe the pattern of IL-10 expression in primary Burkitt's lymphoma (BL) tumor biopsies and in BL lines of defi ned phenotypes, vIL-10 was expressed during the lytic (productive) pha se of EBV infection, but not during virus latency. Although hIL-10 was expressed in the majority of B cell lines, it was not expressed in tw o BL biopsy specimens. Expression of hIL-10 did not correlate with the presence of EBV, but was associated with the differentiation stare of the B cell line. Thus, vIL-10 may enhance the persistence of B cells infected at sites of virus replication, and while hIL-10 may be a fact or in the growth both in vivo and in vitro of some BLs and EBV-transfo rmed B cells, it is not an absolute requirement. (C) 1994 Academic Pre ss, Inc.