A direct immunofluorescent antibody test (DIF) was developed for the r
apid diagnosis of melioidosis, a potentially fatal infection caused by
Pseudomonas pseudomallei. In a clinical evaluation of 369 sputum, pus
, or urine specimens from 272 patients with suspected melioidosis, the
DIF had a sensitivity of 73% and a specificity of 99% compared with c
ulture. Using this DIF, a confident diagnosis of melioidosis can now b
e made within two hours of admission to hospital, compared with the de
lay of two to four days required for culture results. Consequent early
institution of specific antimicrobial therapy may help to save lives.