CHARACTERIZATION OF THE MAIN EGG ENVELOPE PROTEINS OF THE SEA BASS DICENTRARCHUS-LABRAX L (TELEOSTEA, SERRANIDAE)

Fish eggs are surrounded by a resistant acellular coat commonly called the chorion or zona radiata. This study characterizes the eggshell pr oteinaceous content of unfertilized eggs of the sea bass Dicentrarchus labrax, with a view to the preparation of immunogens. Solubilization of the purified eggshells was achieved in 8 M urea followed by one- an d two-dimensional gel electrophoresis. Glycoproteins were detected usi ng concanavalin-A in one and two-dimensional gels, and the principal g lycoproteins had a molecular weight of 47 kDa and 170 kDa. Partial pur ification of a few polypeptides in the 45 kDa to 55 kDa range was achi eved by gel filtration chromatography. Although whole eggshells were r elatively insoluble even in 8 M urea, partial purification of these po lypeptides enable them to dissolve completely in solutions at low ioni c strength. (C) 1994 Wiley-Liss, Inc.