RAPID, NONRADIOACTIVE, AND QUANTITATIVE METHOD TO ANALYZE ZONA-PELLUCIDA MODIFICATIONS IN SINGLE-MOUSE EGGS

Authors
MOOS J KALAB P KOPF GS SCHULTZ RM
Citation
J. Moos et al., RAPID, NONRADIOACTIVE, AND QUANTITATIVE METHOD TO ANALYZE ZONA-PELLUCIDA MODIFICATIONS IN SINGLE-MOUSE EGGS, Molecular reproduction and development, 38(1), 1994, pp. 91-93
Citations number
7
Categorie Soggetti
Reproductive Biology","Developmental Biology",Biology
Journal title
Molecular reproduction and development → ACNP
ISSN journal
1040452X
Volume
38
Issue
1
Year of publication
1994
Pages
91 - 93
Database
ISI
SICI code
1040-452X(1994)38:1<91:RNAQMT>2.0.ZU;2-F
Abstract
A rapid, nonradioactive method to monitor the ZP2 to ZP2(f) conversion in the zona pellucida of single mouse eggs has been developed. This a ssay is based on the chemiluminescent detection of biotinylated ZP2 an d ZP2(f) following electrophoresis under reducing conditions and elect rophoretic transfer to Immobilon P. This method is about 10 times fast er and detects similar ex tents of ZP2 to ZP2(f) conversion following A23187-induced egg activation, when compared to the commonly used radi oiodination procedures. (C) 1994 Wiley-Liss, Inc.