1 KB OF THE LACTASE-PHLORHIZIN HYDROLASE PROMOTER DIRECTS POST-WEANING DECLINE AND SMALL INTESTINAL-SPECIFIC EXPRESSION IN TRANSGENIC MICE

Authors
TROELSEN JT MEHLUM A OLSEN J SPODSBERG N HANSEN GH PRYDZ H NOREN O SJOSTROM H
Citation
Jt. Troelsen et al., 1 KB OF THE LACTASE-PHLORHIZIN HYDROLASE PROMOTER DIRECTS POST-WEANING DECLINE AND SMALL INTESTINAL-SPECIFIC EXPRESSION IN TRANSGENIC MICE, FEBS letters, 342(3), 1994, pp. 291-296
Citations number
29
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
342
Issue
3
Year of publication
1994
Pages
291 - 296
Database
ISI
SICI code
0014-5793(1994)342:3<291:1KOTLH>2.0.ZU;2-C
Abstract
Adult-type hypolactasia is a genetic condition making approximately on e half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestin al-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the inte stinal capacity to hydrolyze lactose. We here demonstrate that the str etch -17 to -994 in the pig LPH-promoter carries cis-elements which di rect a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-re gulation.