The kinetics of enzymatic hydrolysis of rice bran oil in organic solve
nt (n-hexane) by free and immobilized Candida lipolytica lipase were i
nvestigated in a batch reactor. The n-hexane does not alter pH and tem
perature optima, but an increase in n-hexane concentration decreases t
he enzyme activity. The kinetic model proposed to describe the inhibit
ory effect of solvents on lipolysis was found to fit our data well.