CONSTRUCTION OF A TRANSLATION ENHANCER-CONTAINING VECTOR FOR GENE-EXPRESSION IN A PROKARYOTIC 2-CISTRON SYSTEM

A prokaryotic two-cistron system for gene expression, based on the pGE M1 plasmid and containing a translation enhancer in the coding part of the first cistron, has been constructed. The gene to be expressed can be inserted into the vector by means of a PCR-mediated approach using type IIS restriction endonucleases (SDL method). Efficiency of the sy stem is exemplified by the expression of a synthetic gene encoding hum an interleukin 1 alpha.