H-1-NMR THERMODYNAMICAL ANALYSIS OF THE INTERACTIONS OF PROFLAVINE WITH SELF-COMPLEMENTARY DEOXYTETRANUCLEOTIDES OF DIFFERENT BASE SEQUENCE

Enthalpies and entropies of complex formation (1:1, 2:1, 1:2, and 2:2 complexes) between proflavine and tetranucleotides of different base s equence have been determined by 500 MHz proton NMR chemical shifts, en abling the contributions to be differentiated for the formation of dif ferent types of complexes in solution. Comparison of the calculated th ermodynamical parameters has lead to an understanding of the nature of the intermolecular interactions responsible for the formation of dye complexes with the different tetranucleotides.