AN ISOFORM OF THE HUMAN CALCITONIN RECEPTOR IS EXPRESSED IN TT CELLS AND IN MEDULLARY CARCINOMA OF THE THYROID

Citation
Jl. Frendo et al., AN ISOFORM OF THE HUMAN CALCITONIN RECEPTOR IS EXPRESSED IN TT CELLS AND IN MEDULLARY CARCINOMA OF THE THYROID, FEBS letters, 342(2), 1994, pp. 214-216
Citations number
13
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
00145793
Volume
342
Issue
2
Year of publication
1994
Pages
214 - 216
Database
ISI
SICI code
0014-5793(1994)342:2<214:AIOTHC>2.0.ZU;2-D
Abstract
We amplified, using the polymerase chain reaction and calcitonin recep tor (CTR) specific primers, RNA extracted from medullary thyroid carci noma (MTC) and the derived TT cell line. Both secrete large amounts of calcitonin. Electrophoresis of amplification products revealed, in bo th cases, an ethidium bromide-stained band that hybridized to a CTR pr obe. Sequencing the band amplified from TT cells revealed an open read ing frame identical to the sequence of H-CTR but lacking 16 amino acid s in the first intracellular loop. This demonstrates the existence of an mRNA coding for a subtype of H-CTR which is expressed in TT cells a nd MTC.