PREPARATIVE-SCALE CULTURE OF ESCHERICHIA-COLI-CELLS EXPRESSING THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TAT PROTEIN

Authors
MCKENNA MC MUCHARDT C GAYNOR R EISENBERG D
Citation
Mc. Mckenna et al., PREPARATIVE-SCALE CULTURE OF ESCHERICHIA-COLI-CELLS EXPRESSING THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TAT PROTEIN, Protein expression and purification, 5(2), 1994, pp. 105-111
Citations number
21
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
Protein expression and purification → ACNP
ISSN journal
10465928
Volume
5
Issue
2
Year of publication
1994
Pages
105 - 111
Database
ISI
SICI code
1046-5928(1994)5:2<105:PCOEET>2.0.ZU;2-0
Abstract
A procedure leading to a 100-liter fermenter culture of Escherichia co li cells expressing the human immunodeficiency virus type 1 (HIV-1) tr ans-activator (Tat) protein is described. The effects of growth temper ature and of cell density at the time of induction on the yield of Tat were investigated. Tat was identified by SDS-gel electrophoresis and Western blot. Tat represents approximately 10% of the soluble protein in the cell lysate. (C) 1994 Academic Press, Inc.