CHANGES IN MANNOSE CONTENT IN CELLULAR POLYSACCHARIDES OF SPOROBOLOMYCES-HOLSATICUS DURING EXOMANNAN BIOSYNTHESIS

Carbohydrate composition of soluble and insoluble fractions of cell wa lls of Sporobolomyces holsaticus yeast during extracellular mannan bio synthesis was studied. The content of mannose and glucose in insoluble structural component was found to be permanent. An increase of mannos e content in the soluble component of cell walls from 16.4 to 47.6% co rrelated with extracellular mannan release into culture fluid. Electro n microscopic investigations have shown that during maximum synthesis of the exopolysaccharide the cell wall thickness increased by about te n-fold due to significant accumulation of substances of carbohydrate n ature.