OPTIMIZATION OF BACILLUS-INTERMEDIUS CULTIVATION CONDITIONS FOR OBTAINING RIBONUCLEASE LABELED BY STABLE ISOTOPES

Authors
ZNAMENSKAYA LV VERSHININA VI KUROCHKIN AV KRASNOV SI LESHCHINSKAYA IB
Citation
Lv. Znamenskaya et al., OPTIMIZATION OF BACILLUS-INTERMEDIUS CULTIVATION CONDITIONS FOR OBTAINING RIBONUCLEASE LABELED BY STABLE ISOTOPES, Microbiology, 62(4), 1993, pp. 420-422
Citations number
9
Categorie Soggetti
Microbiology
Journal title
Microbiology → ACNP
ISSN journal
00262617
Volume
62
Issue
4
Year of publication
1993
Pages
420 - 422
Database
ISI
SICI code
0026-2617(1993)62:4<420:OOBCCF>2.0.ZU;2-2
Abstract
Two versions of medium composition for obtaining of binase labelled by C-13 or N-15 were selected through a multifactor experiment. A medium containing 0.8% glucose and 2.5% hydrolysate of Anabaena sp. as carbo n and nitrogen sources, respectively, is recommended for production of N-15-labelled enzyme and medium containing hydrolysate as the sole ca rbon and nitrogen source is recommended for production of C-13-labelle d enzyme. Addition of 0.125 mu g/ml of actinomycin D to the medium inc reases the enzyme yield.