A lysine sensor for process control of lysine fermentation was develop
ed based on a Clark-type electrode in combination with L-lysine-alpha-
oxidase. The enzyme, isolated from Trichoderma viride, was immobilized
between a cellulose and a polypropylene foil using a polyurethane res
in. Lysine determinations were carried out in a flow-through system as
anodic measurements when H2O2 was measured and as cathodic measuremen
ts when the consumption of O2 was followed. The sensitivity of the sen
sor toward other amino acids was determined.