A LYSINE SENSOR FOR PROCESS-CONTROL

Authors
SIEGLER K WEBER B WEBER E TONDER K KLINGNER E RIECHERT F
Citation
K. Siegler et al., A LYSINE SENSOR FOR PROCESS-CONTROL, Journal of chemical technology and biotechnology, 59(3), 1994, pp. 279-287
Citations number
14
Categorie Soggetti
Engineering, Chemical",Chemistry,"Biothechnology & Applied Migrobiology
Journal title
Journal of chemical technology and biotechnology → ACNP
ISSN journal
02682575
Volume
59
Issue
3
Year of publication
1994
Pages
279 - 287
Database
ISI
SICI code
0268-2575(1994)59:3<279:ALSFP>2.0.ZU;2-F
Abstract
A lysine sensor for process control of lysine fermentation was develop ed based on a Clark-type electrode in combination with L-lysine-alpha- oxidase. The enzyme, isolated from Trichoderma viride, was immobilized between a cellulose and a polypropylene foil using a polyurethane res in. Lysine determinations were carried out in a flow-through system as anodic measurements when H2O2 was measured and as cathodic measuremen ts when the consumption of O2 was followed. The sensitivity of the sen sor toward other amino acids was determined.