IMMOBILIZATION OF BETA-GALACTOSIDASE ONTO GELATIN BY GLUTARALDEHYDE AND CHROMIUM(III) ACETATE

Authors
SUNGUR S AKBULUT U
Citation
S. Sungur et U. Akbulut, IMMOBILIZATION OF BETA-GALACTOSIDASE ONTO GELATIN BY GLUTARALDEHYDE AND CHROMIUM(III) ACETATE, Journal of chemical technology and biotechnology, 59(3), 1994, pp. 303-306
Citations number
14
Categorie Soggetti
Engineering, Chemical",Chemistry,"Biothechnology & Applied Migrobiology
Journal title
Journal of chemical technology and biotechnology → ACNP
ISSN journal
02682575
Volume
59
Issue
3
Year of publication
1994
Pages
303 - 306
Database
ISI
SICI code
0268-2575(1994)59:3<303:IOBOGB>2.0.ZU;2-L
Abstract
Beta-Galactosidase (EC 3.2.1.23) was immobilised onto a gelatin carrie r system by crosslinking. Glutaraldehyde and chromium(III) acetate wer e used as crosslinking agents. The effect of pH on enzyme activity, ki netic parameters and reusability of the immobilised enzyme were invest igated. To obtain a better relative activity, a method was developed a nd further modified to increase the surface area of the support system by using polyacrylamide. Relative activity enhancement of 68% was ach ieved in the gelatin-glutaraldehyde system.