ONE-MEGABASE YEAST ARTIFICIAL CHROMOSOME AND 400-KILOBASE COSMID-PHAGE CONTIGS CONTAINING THE VON HIPPEL-LINDAU TUMOR-SUPPRESSOR AND CA2-TRANSPORTING ADENOSINE-TRIPHOSPHATASE ISOFORM-2 GENES()

We have isolated and ordered yeast artificial chromosomes (YACs) and c osmids surrounding the von Hippel-Lindau (VHL) tumor suppressor and pl asma membrane Ca2+-transporting ATPase isoform 2 (PMCA-2) genes on chr omosome 3p25-26. The YAC contig consists of six YACs and covers a regi on of 1 megabase. A cosmid-phage contig around VHL and PMCA-2 genes (4 00 kilobases) was established and integrated into the YAC map. Using t hese clones, we generated an EcoRI map of the 400-kilobase region. PMC A-2 and VHL complementary DNA were positioned entirely within the cosm id-phage contig as well as two polymorphic markers (D3S601 and D3S1317 ). This physical map of the cloned region will allow a detailed analys is of both the PMCA-2 and VHL genes. Some of the genomic clones may be useful for isolation of the full-length VHL complementary DNA.