Wheat proembryos were excised 7 days after anthesis and germinated to
normal fertile giants with an efficiency of 90%. These embryos had cle
arly differentiated shoot apical meristems and represent an interestin
g model system for studies of meristem biology. A combination of two m
edia with different osmolarities allowed the continued development of
the embryos before induction of germination. Normal germination occurr
ed only after the first leaf primordium had already covered the shoot
apical meristem.