ISOLATION AND VIRAL-INFECTION OF CAPSICUM LEAF PROTOPLASTS

Authors
MURPHY JF KYLE MM
Citation
Jf. Murphy et Mm. Kyle, ISOLATION AND VIRAL-INFECTION OF CAPSICUM LEAF PROTOPLASTS, Plant cell reports, 13(7), 1994, pp. 397-400
Citations number
17
Categorie Soggetti
Plant Sciences
Journal title
Plant cell reports → ACNP
ISSN journal
07217714
Volume
13
Issue
7
Year of publication
1994
Pages
397 - 400
Database
ISI
SICI code
0721-7714(1994)13:7<397:IAVOCL>2.0.ZU;2-C
Abstract
A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plan ts and the concentration of mannitol used as osmoticum. Protoplasts is olated from each of the genotypes became infected when inoculated via electroporation with viral RNA from either pepper mottle potyvirus, to bacco etch potyvirus or cucumber mosaic cucumovirus.