ENZYMATIC-SYNTHESIS OF TRITIUM-LABELED PROSTAGLANDIN D-2 AND ITS CONVERSION TO OTHER PROSTAGLANDINS

The one-stage enzymatic synthesis of tritium-labelled prostaglandin D- 2 from labelled arachidonic acid was performed by using the enzyme sys tem PGH-synthetase/PGH-PGD-isomerase. By enzymatic and chemical transf ormation of H-3PGD(2) the following compounds were obtained: 15-keto -13, 14-dihydro-H-3PGD(2), 9 alpha,11 beta-H-3PGF(2), 9-deoxy-Delt a(9)-H-3-PGD(2) (H-3PGJ(2)) and Delta(12)-13, 14-dihydro-H-3PGJ( 2). It was found that L-selectride is a more effective reducing agent than sodium borohydride in the synthesis of 9 alpha,11 beta-H-3PGF(2 ).