SYNTHESIS OF [4,5-H-3-LEU(4)]SALMON CALCITONIN

The synthesis of salmon calcitonin, a C-terminal amide dotriacontapept ide, labelled with tritium in the leucine residue at position 4 to a s pecific activity of 100Ci mmole(-1) is described. The peptide was asse mbled using the Fmoc-Polyamide solid-phase synthesis strategy incorpor ating tritium labelled leucine, in the form of Fmoc4,5-H-3-leucine-pen tanuorophenylester, at the appropriate stage. The Fmoc4,5-H-3-leucine- pentanuorophenylester was prepared via a multistage synthesis from 4,5 -dehydroleucine.