PRESYNAPTIC AND POSTSYNAPTIC MODULATORS OF EXCITATORY NEUROTRANSMISSION - COMPARATIVE EFFECTS ON HYPOXIA HYPOGLYCEMIA IN CORTICAL CULTURES/

Authors
KORETZ B AHERN KV WANG N LUSTIG HS GREENBERG DA
Citation
B. Koretz et al., PRESYNAPTIC AND POSTSYNAPTIC MODULATORS OF EXCITATORY NEUROTRANSMISSION - COMPARATIVE EFFECTS ON HYPOXIA HYPOGLYCEMIA IN CORTICAL CULTURES/, Brain research, 643(1-2), 1994, pp. 334-337
Citations number
28
Categorie Soggetti
Neurosciences
Journal title
Brain research → ACNP
ISSN journal
00068993
Volume
643
Issue
1-2
Year of publication
1994
Pages
334 - 337
Database
ISI
SICI code
0006-8993(1994)643:1-2<334:PAPMOE>2.0.ZU;2-C
Abstract
Neuron-enriched cultures from embryonic rat cerebral cortex were expos ed to hypoxia and hypoglycemia, and the resulting cellular injury was quantified by measuring lactate dehydrogenase (LDH) release, which was maximal after 20-24 h. The increase in LDH release produced by hypoxi a/hypoglycemia was prevented by N-methyl-D-aspartate (NMDA) antagonist s, but not by three classes of drugs thought to modulate glutamate rel ease: Ca2+ channel antagonists (nimodipine, omega-conotoxin GVIA, omeg a-agatoxin-IVA), K-ATP channel activators (cromakalim, diazoxide), and glutamate transport inhibitors (dihydrokainate, DL-threo-beta-hydroxy aspartate).