THE TERT-BUTYLOXYCARBONYL-(BOC) GROUP, A SUITABLE 2'-OH-PROTECTING GROUP IN OLIGORIBONUCLEOTIDE SOLID-PHASE SYNTHESIS

Authors
LOOSE G NAUMANN W WINKLER A SUPTITZ G
Citation
G. Loose et al., THE TERT-BUTYLOXYCARBONYL-(BOC) GROUP, A SUITABLE 2'-OH-PROTECTING GROUP IN OLIGORIBONUCLEOTIDE SOLID-PHASE SYNTHESIS, Journal fur praktische Chemie, Chemiker-Zeitung, 336(3), 1994, pp. 233-236
Citations number
10
Categorie Soggetti
Chemistry,"Chemistry Applied
Journal title
Journal fur praktische Chemie, Chemiker-Zeitung → ACNP
ISSN journal
09411216
Volume
336
Issue
3
Year of publication
1994
Pages
233 - 236
Database
ISI
SICI code
0941-1216(1994)336:3<233:TTGAS2>2.0.ZU;2-4
Abstract
The solid-phase synthesis of the dodecaribonucleotide ACCACUAAAGCG is described using the tert. butyloxycarbonyl (Boc) as 2'-OH-protecting g roup. The synthesis was carried out on a filter disc support Whatman 3 MM by the phosphotriester method. Under these conditions, the 2'-OH-B oc-group proved to be absolutely stable. Moreover, it is quantitativel y and selectively removable by 4n HCl/dioxane. Thus, the group renders to be an excellent persistent 2'-OH-protection in ribonucleotide synt hesis.