ENANTIOSELECTIVE DETERMINATION OF DIPRAFENONE IN HUMAN PLASMA

A sensitive (10.0 ng/ml) and stereoselective HPLC method for analysis of S(-)- and R(+)-dipraferone in human plasma has been developed. The assay is based on derivatization with homochiral R(-)-1-(1-naphthyl)et hyl isocyanate to give the diastereomeric derivates and their separati on and quantitation using HPLC with UV-detection (220 nm). Details of this stereoselective assay procedure which has already been applied to the multisample analyses from a human pharmacokinetic study are descr ibed. The data obtained indicated that the stereoselective disposition of diprafenone enantiomers is quite different from their structural a nd pharmacological analog propafenone.