Ho. Saridakis et al., VIRULENCE PROPERTIES OF ESCHERICHIA-COLI STRAINS BELONGING TO ENTEROPATHOGENIC (EPEC) SEROGROUPS ISOLATED FROM CALVES WITH DIARRHEA, Veterinary microbiology, 54(2), 1997, pp. 145-153
Nineteen Escherichia coli strains belonging to enteropathogenic (EPEC)
serogroups were isolated from calves with diarrhea in Parana State, B
razil, and studied for virulence markers associated with EPEC or enter
ohemorrhagic E. coli (EHEC). The 19 isolates belonged to 12 serotypes
with isolates of O26:H11, O119:H25 and O114:H- being the most prevalen
t. Localized adherence (LA) was demonstrated for 37% of the isolates,
consisting of all four O26:H11, both O114:H- and one O114:H40 isolates
. All the LA strains were positive in the fluorescent-actin staining (
FAS) test and possessed attaching-effacing E. coli (eae) sequences, bu
t only O114 strains hybridized with the EPEC adherence factor (EAF) pr
obe. None of the strains produced Shiga-like toxins (Verotoxin). Only
the O26:H11 strains hybridized with the EHEC plasmid specific (CVD419)
probe and were enterohemolytic, properties associated with EHEC strai
ns. This investigation demonstrates that among the bovine strains isol
ated only those of serogroup O114 behaved as typical EPEC.