MULTILOCUS ENZYME ELECTROPHORESIS USING ULTRATHIN POLYACRYLAMIDE GELS

Authors
JOHN MA HUSSAIN Z
Citation
Ma. John et Z. Hussain, MULTILOCUS ENZYME ELECTROPHORESIS USING ULTRATHIN POLYACRYLAMIDE GELS, Journal of microbiological methods, 19(4), 1994, pp. 307-313
Citations number
20
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
19
Issue
4
Year of publication
1994
Pages
307 - 313
Database
ISI
SICI code
0167-7012(1994)19:4<307:MEEUUP>2.0.ZU;2-V
Abstract
Multilocus enzyme electrophoresis (MEE) using starch gels has been wid ely used to study bacterial genetics and for epidemiological typing. W e describe a technique using ultrathin polyacrylamide gel electrophore sis (PAGE) which gives improved resolution and reproducibility, decrea sing the number of gels which have to be run. We also describe a techn ique whereby several enzymes may be developed from a single running ge l, resulting in significant time savings.