D. Horiguchi et al., A NOVEL TIME-RESOLVED FLUOROIMMUNOASSAY USING A MACROCYCLIC EUROPIUM LIGAND AS A LABEL, Chemical and Pharmaceutical Bulletin, 42(4), 1994, pp. 972-975
A novel macrocyclic ligand for Eu3+ ,4,7,10,13,16]hexaazacyclooctadeci
ne-8,19-diacetic acid) was used to label an anti-human chorionic gonad
otropin (hCG) monoclonal antibody. The time-resolved fluoroimmunoassay
of hCG using this labeled antibody showed a linear response over the
range 0.1 to 10 I.U. hCG/ml.