T. Psenakova et S. Kasiarova, THE VANADATE-SENSITIVE ATPASE FROM PLASMA-MEMBRANE OF MAIZE ROOTS, Acta Societatis Botanicorum Poloniae, 62(3-4), 1993, pp. 171-173
Plasma membrane vesicles isolated from com roots were shown to possess
Mg2+-dependent and K+-stimulated ATPase activity, which was inhibited
by vanadate, insensitive to nitrate and molybdate, and very little se
nsitive to azide. The optimum of phosphohydrolase activity was at pH 6
.5 and temperature optimum 40-degrees-C. ATP was the preferred substra
te. The Plasma membrane ATPase was reconstituted into soybean phosphol
ipid liposomes.