CLONING AND CHARACTERIZATION OF A CHROMOSOMAL GENE-CLUSTER, PAH, THATENCODES THE UPPER PATHWAY FOR PHENANTHRENE AND NAPHTHALENE UTILIZATION BY PSEUDOMONAS-PUTIDA OUS82

Authors
KIYOHARA H TORIGOE S KAIDA N ASAKI T IIDA T HAYASHI H TAKIZAWA N
Citation
H. Kiyohara et al., CLONING AND CHARACTERIZATION OF A CHROMOSOMAL GENE-CLUSTER, PAH, THATENCODES THE UPPER PATHWAY FOR PHENANTHRENE AND NAPHTHALENE UTILIZATION BY PSEUDOMONAS-PUTIDA OUS82, Journal of bacteriology, 176(8), 1994, pp. 2439-2443
Citations number
32
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
176
Issue
8
Year of publication
1994
Pages
2439 - 2443
Database
ISI
SICI code
0021-9193(1994)176:8<2439:CACOAC>2.0.ZU;2-U
Abstract
A 25-kb DNA Sail fragment cloned from the chromosomal DNA of Pseudomon as putida OUS82, which utilizes phenanthrene (Phn(+)) and naphthalene (Nah(+)), carried all of the genes necessary for upper naphthalene cat abolism. Cosmid recombinant pIP7 complemented both the Nah(-) and Phn( -) defects of OUS8211 (Trp(-) Nah(-) Phn(-) Sal(+) [salicylate utilizi ng] Hna(+) [1-hydroxy-2-naphthoate utilizing]) and only the Phn(-) def ect of OUS8212 (Trp(-) Nah(-) Phn(-) Sal(-) Hna(+)). The results indic ate that strain OUS82 uses different pathways after o-hydroxycarboxyli c aromatics in the catabolism of naphthalene and phenanthrene.