Mv. Reddy et al., RAPID ASSESSMENT OF MYCOBACTERIAL GROWTH INSIDE MACROPHAGES AND MICE,USING THE RADIOMETRIC (BACTEC) METHOD, Tubercle and lung disease, 75(2), 1994, pp. 127-131
Setting: Conventional methods of determining the growth and drug susce
ptibility of mycobacteria either in macrophages or in vivo are time co
nsuming and laborious. Objective: We sought to develop a simple and ra
pid method for assessing growth and susceptibility of mycobacteria in
macrophages and in vivo. Design: The radiometric (BACTEC) method was c
ompared with the conventional method for rapid assessment of growth an
d susceptibility of Mycobacterium avium complex (MAC) to clofazimine (
CFM) and its two analogues (B4100 and B4101) in macrophages and in bei
ge mice. Results and conclusions: A linear relationship was observed b
etween growth index (GI) readings by the BACTEC method and the colony
forming unit (CFU) counts obtained by conventional plating on 7H11 aga
r in vitro of lysates from infected macrophages and of homogenates fro
m the infected organs. There was also a good agreement between the two
methods with respect to susceptibility of MAC to CFM and its analogue
s both in macrophages and in spleen. The BACTEC method, in addition to
being quick and simple, was found to be more consistent. Our studies
thus demonstrate that the radiometric method has potential application
in rapid screening of drugs for antimycobacterial activity in macroph
ages and in animals besides its established value in in vitro screenin
g.