ELECTROGENERATED CHEMILUMINESCENCE DETECTION FOR CAPILLARY ELECTROPHORESIS

Citation
Sd. Gilman et al., ELECTROGENERATED CHEMILUMINESCENCE DETECTION FOR CAPILLARY ELECTROPHORESIS, The Journal of microcolumn separations, 6(2), 1994, pp. 97-106
Citations number
42
Categorie Soggetti
Chemistry Analytical
ISSN journal
10407685
Volume
6
Issue
2
Year of publication
1994
Pages
97 - 106
Database
ISI
SICI code
1040-7685(1994)6:2<97:ECDFCE>2.0.ZU;2-R
Abstract
Electrogenerated chemiluminescence (ECL) detection based on the reacti on of luminol and H2O2 has been developed for capillary electrophoresi s. The ECL response at carbon fiber and platinum wire microelectrodes has been investigated. Carbon fiber electrodes are observed to be less sensitive than platinum electrodes but provide a more stable response . The ECL response at a carbon fiber electrode is linear over three or ders of magnitude for luminol with a linear correlation coefficient of 0.998. Mass detection limits of 92 amol and 260 amol have been obtain ed for luminol using platinum wire microelectrodes and carbon fiber mi croelectrodes, respectively. The influence of hydrogen peroxide concen tration (in the detection buffer reservoir) on the luminescence respon se has been examined and found to be dependent on electrode type. The dependence of the ECL response on the applied voltage at the microelec trode has been studied for both electrode types. Capillary electrophor esis with ECL detection has been used for analysis of amines derivatiz ed with N-(4-aminobutyl)-N-ethylisoluminol coupled to N,N-disuccinimid ylcarbonate (ABEI-DSC). Detection limits of 2.0 fmol and 0.96 fmol are obtained for n-octylamine and n-propylamine, respectively. In additio n, ABEI-DSC has been used to successfully label the tripeptide Val-Tyr -Val, and micellar electrokinetic capillary chromatography has been us ed with ECL detection for the separation of ABEI-DSC labeled amines.