Sd. Gilman et al., ELECTROGENERATED CHEMILUMINESCENCE DETECTION FOR CAPILLARY ELECTROPHORESIS, The Journal of microcolumn separations, 6(2), 1994, pp. 97-106
Electrogenerated chemiluminescence (ECL) detection based on the reacti
on of luminol and H2O2 has been developed for capillary electrophoresi
s. The ECL response at carbon fiber and platinum wire microelectrodes
has been investigated. Carbon fiber electrodes are observed to be less
sensitive than platinum electrodes but provide a more stable response
. The ECL response at a carbon fiber electrode is linear over three or
ders of magnitude for luminol with a linear correlation coefficient of
0.998. Mass detection limits of 92 amol and 260 amol have been obtain
ed for luminol using platinum wire microelectrodes and carbon fiber mi
croelectrodes, respectively. The influence of hydrogen peroxide concen
tration (in the detection buffer reservoir) on the luminescence respon
se has been examined and found to be dependent on electrode type. The
dependence of the ECL response on the applied voltage at the microelec
trode has been studied for both electrode types. Capillary electrophor
esis with ECL detection has been used for analysis of amines derivatiz
ed with N-(4-aminobutyl)-N-ethylisoluminol coupled to N,N-disuccinimid
ylcarbonate (ABEI-DSC). Detection limits of 2.0 fmol and 0.96 fmol are
obtained for n-octylamine and n-propylamine, respectively. In additio
n, ABEI-DSC has been used to successfully label the tripeptide Val-Tyr
-Val, and micellar electrokinetic capillary chromatography has been us
ed with ECL detection for the separation of ABEI-DSC labeled amines.