V. Enzmann et K. Drossler, IMMUNOHISTOCHEMICAL DETECTION OF SUBSTANCE-P AND VASOACTIVE-INTESTINAL-PEPTIDE FIBERS IN THE AURICULAR LYMPH-NODES OF SENSITIZED GUINEA-PIGS AND MICE, Acta histochemica, 96(1), 1994, pp. 15-18
The presence of substance P (SP) and vasoactive intestinal peptid (VIP
) in auricular lymph nodes of normal and sensitized guinea pigs and mi
ce has been investigated using polyclonal antibodies directed against
these neurotransmitters and the unlabelled peroxidase-antiperoxidase p
rocedure. Positive immunoreactivity could be observed only in draining
lymph nodes of sensitized animals. SP-immunoreactivity was found in m
ice and guinea pigs but a VIP-positive reaction only in mice. The immu
nopositive sites were always associated with blood vessels. The presen
t study suggests that the neurotransmitters SP and VIP are involved in
mechanisms of sensitization taking place in secondary lymphoid organs
.