NONINVASIVE SAMPLING FOR DETECTION OF GENITAL-INFECTION WITH CHLAMYDIA-TRACHOMATIS IN MALES UTILIZING URINARY LEUKOCYTE ESTERASE TESTS AND IMMUNOASSAYS
Ma. Domeika et al., NONINVASIVE SAMPLING FOR DETECTION OF GENITAL-INFECTION WITH CHLAMYDIA-TRACHOMATIS IN MALES UTILIZING URINARY LEUKOCYTE ESTERASE TESTS AND IMMUNOASSAYS, Infection, 22(2), 1994, pp. 65-68
First-void urine specimens from 224 male recruits and 443 patients of
venereal disease clinics without complaints of symptoms of urethritis
were collected. Urinary leukocyte esterase test, two enzyme immunoassa
ys (EIAs: Syva MicroTrak and Orion), a chemi luminometric assay (Magic
Lite) and Syva's MicroTrak direct immunofluorescence test were used.
The prevalence of chlamydial urethritis in the study population as det
ermined by direct immunofluorescence test of first-void urine in the m
ilitary recruits and venereal disease patients was 1.3% and 6.3%, resp
ectively. The denominator used for calculation of sensitivities was th
e Sum of patients with positive test results in at least two of the di
fferent test systems used. The sensitivities of first-void urine were
100% for Syva EIA, 96.7% for Orion ELA and 86.7% for the chemiluminome
tric assay. All assays proved highly specific (99.5-99.7%). Compared w
ith direct immunofluorescence test of first-void urine, the urine leuk
ocyte esterase test had a sensitivity of 93.6% and a specificity of 94
.3%. The study showed that the urine leukocyte esterase test is an eff
ective method to detect males infected by Chlamydia trachomatis.