PROTEIN GENE-PRODUCT 9.5-IMMUNOREACTIVE RETINAL NEURONS IN NORMAL DEVELOPING RATS AND RATS WITH OPTIC-NERVE OR TRACT LESION

The present immunocytochemical study indicates that protein gene produ ct 9.5 (PGP 9.5) in the rat retina first appears in a population of ne urons in the inner and central part of the neuroblast layer at embryon ic day (E) 14. Presumptive horizontal cells which are PGP 9.5 positive were observed at E17. At birth, cells in the inner nuclear layer and ganglion cell layer (GCL) as well as the inner plexiform layer (IPL) w ere positive. Further differentiation, particularly the appearance and the formation of immunoreactive sublaminae in the IPL, was observed i n the first 2 postnatal weeks. This pattern reached adult levels by po stnatal day 14. In rats with unilateral neonatal optic tract lesion or ; optic nerve transection as young adults, 43-45% of the immunoreactiv e cells were lost in the GCL. However, only minor changes were detecte d in the IPL, suggesting that amacrine cells contribute mainly to the PGP 9.5 immunoreactivity in this laminar zone of the retina.