EFFECTS OF FLUID, SHEAR-STRESS ON BONE-CELLS

Citation
Jl. Williams et al., EFFECTS OF FLUID, SHEAR-STRESS ON BONE-CELLS, Biorheology, 31(2), 1994, pp. 163-170
Citations number
27
Categorie Soggetti
Biophysics,"Engineering, Biomedical
Journal title
ISSN journal
0006355X
Volume
31
Issue
2
Year of publication
1994
Pages
163 - 170
Database
ISI
SICI code
0006-355X(1994)31:2<163:EOFSOB>2.0.ZU;2-U
Abstract
Neonatal rat calvarial cells (osteoblast-enriched) were cultured in mo nolayer on glass slides and subjected to a step increase in shear stre ss ranging from 3 to 60 dyne/cm(2) in a laminar flow chamber. The leve l of intracellular free calcium ion concentration was monitored using Fura-2 fluorescence during the application of stress. Application of a step increase in stress resulted in a transient increase in calcium s tarting about 10-20 s after turning on the pump, reaching a peak in ab out 35 s and declining thereafter, despite the continued shear stress, and reaching near baseline values in about 100 s. No change in cytopl asmic calcium was observed with a step increase in shear stress of 3 d yne/cm(2) Between 6 dyne/cm(2) and 60 dyne/cm(2) the magnitude of the calcium response increased with the applied shear stress.